Regulatory

Part:BBa_M36543

Designed by: Anna McGregor, Maya Anjur-Dietrich, and Kay Hung   Group: Stanford BIOE44 - S11   (2012-12-07)


Calcium Induced Promoter

This part is the promoter of the human prolactin (PRL) gene, which has been shown to be activated by increased levels of Ca2+ in cells. As there is little conclusive information about mammalian promoters other than they can be found in genetic control regions (usually upstream of the target gene), the genetic sequence was taken from the 2000 bases upstream of PRL in the human genome. Since this selection included a TATAA box, indicative of promoters that initiate at specific sites, it was possible to infer the presence of the PRL promoter.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 82
    Illegal EcoRI site found at 179
    Illegal EcoRI site found at 367
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 1732


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Parameters
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